Universal Mobile IACE POC Biomarker Detection
Ubiquitous Biomarker Diagnostics Accessible and Affordable by All
The universal Internet cloud based IACE biomarker detection system will not only revolutionize healthcare by early detection of disease precursors but provide a platform for optimizing health
Ubiquitous Biomarker Diagnostics Accessible and Affordable by All
The universal Internet cloud based IACE biomarker detection system will not only revolutionize healthcare by early detection of disease precursors but provide a platform for optimizing health.
Proven Results of Concentration of Immunoaffinity Plus Selectivity of Capillary Electrophoresis
The All Inclusive IACE Device
Integrating Several Component Functionalities into a Single Portable Instrument
Item #1
Lorem
ImmunoAffinity
IACE Steps
Steps of on-line sample immunoaffinity preconcentration before CE.
(A) Sample introduction and binding of analytes to the immobilized affinity ligands localized within the ACM device,
(B) washing of the excess amount of matrix containing salts and unwanted materials,
(C) introduction of a plug of an elution buffer or solution,
(D) introduction of separation buffer to push the plug of elution buffer, and
(E) initiation of the process of separation using an appropriate buffer.
The capillary or channel can be connected to one or more detectors, preferentially UV, LIF and MS, for the quantification,
identification and characterization of the separated analytes.
CE
IACE Steps
Unidirectional vs OrthodirectionalIACE
Schematic diagram of an on-line immunocapture device containing one or more affinity selectors in immunoaffinity capillary
electrophoresis technology (IACE), prior to CE separation and detection of the captured and eluted analytes.
(A) A selector device format, known as linear or unidirectional analyte concentrator-microreactor (ACM) device or area.
(B) A selector device format, known as orthogonal ACM device or area.
The area where an affinity ligand is immobilized has been reported in the literature as the solid-phase extraction (SPE)
analyte preconcentrator area, or the analyte concentrator microreactor (ACM) device or area.
Orthogonal IACE with Steps
Orthogonal IACE with Steps
Orthogonal IACE with Steps
Demonstration of Selection Capability of IACE for Isoforms vs ELISA
Depicts a schematic representing the main differences betrween the traditional mono-dimensional sandwich ELISA technique with the two-dimensional irnmuncapture-separation IACE. The two-dimensional IACE technology can achieve the capture, separation, and partial characterization of each individual isoform.
In contrast, the ELISA is just a single result unable to distinguish molecular differences of the Isoforms.
Source: p.17 Biomolecules 2021, t11, 1443